Occurrence of Lectin in the Silkgland of the Silkworm, Bombyx mori |
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Authors: | Kazuhito Amanai Mie Sakai Sho Sakurai Toshio Mori Osamu Nikaido Tetsuya Ohtaki |
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Affiliation: | Department of Biology, Faculty of Science, Kanazawa University, Kanazawa 920, Japan.;Division of Radiation Biology, Faculty of Pharmaceutical Science, Kanazawa University, Kanazawa 920, Japan |
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Abstract: | Monoclonal antibodies were prepared against the 350 kDa lectin purified from larval hemolymph of the silkworm, Bombyx mori . The antibodies inhibited the hemagglutinating activity (HA activity) and bound specifically to the hemolymph 350 kDa lectin on Western blotting analysis. Immunohistological observations revealed the occurrence of lectin in the cuticular intima of the anterior silk gland, but not the middle or posterior silk glands of fifth instar larvae of Bombyx mori . Extracts from the anterior silk glands showed HA activity and exhibited the same biochemical characteristics as those of the 350 kDa lectin in the hemolymph. These results suggested that lectin-like molecules in epithelial tissues may be important in histolysis during molting and metamorphosis. |
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