Prolactin binding sites in human erythrocytes and lymphocytes |
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| Authors: | G Bellussi G Muccioli C Ghè R Di Carlo |
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| Institution: | 1. Institute of Pharmacology, University of Turin, Corso Raffaello 30, 10125 Turin, Italy;2. Department of Experimental Pharmacology, University of Naples, via Rodinò 22, 80138 Naples, Italy;1. School of Marine Sciences, Ningbo University, Ningbo, 315211, China;2. Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, 315211, China;2. Laboratory of Physiological Chemistry, de Duve Institute and Université catholique de Louvain, 1200 Brussels, Belgium;1. Jiangsu Province Blood Center, China;2. School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, China;3. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, China |
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| Abstract: | Specific binding sites for prolactin (PRL) have been studied in human peripheral lymphocytes and erythrocytes of normal adult volunteers and of term cord bloods. In erythrocytes from healthy adult subjects of both sexes a very low specific binding of 125I-human PRL was found (0.24%), whereas a higher binding was found in term cord blood (1.1%). The binding was hormone specific, the binding capacity was 2.6 fmol/4 X 10(9) cells and the Kd was 3.4 X 10(-10) M. In lymphocytes of both adults and term cord bloods an evident specific binding was observed (male adults: 1.6%; female adults: 1.7%; cord blood: 1.8%). The binding was specific for lactogenic hormones and the binding capacity was 3.7 fmol/2 X 10(6) cells and the Kd was 3.9 X 10(-10) M. The presence of specific binding sites for PRL on human erythrocytes and lymphocytes could be used to study PRL binding on blood cells of patients in different physiological or pathological situations. |
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