Purification and characterization of two serine protease inhibitors from the hemolymph of Mythimna unipuncta |
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Authors: | Cherqui A Cruz N Simões N |
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Affiliation: | Departamento da Biologia, Universidade dos A?ores, Rua M?e Deus, 9500 Ponta Delgada, Portugal. anas.cherqui@u-picardie.fr |
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Abstract: | Two serine protease inhibitors, trypsin inhibitor and alpha-chymotrypsin inhibitor, were isolated from the hemolymph of Mythimna unipuncta. Mythimna trypsin and alpha-chymotrypsin inhibitors were purified by gel filtration and anion-exchange chromatography. They displayed molecular masses of 52 kDa and 43 kDa, respectively, as determined by electrophoresis under reducing and non-reducing conditions on denaturing polyacrylamide gels. Their isoelectric points were evaluated by isoelectric focusing and two-dimensional electrophoresis. Their N-terminal sequences have been analyzed as APSDTTIAETLTITEEFFPD and FDESFGFQGPSTYEKTPLGEP, respectively. The role of these inhibitors in the regulation of the defense reaction of the insect is discussed. |
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