| r－干扰素对喉癌细胞系HEP－2HLA－DR抗原和增殖细胞核抗原表达的影响 |
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| 引用本文： | 阮清伟,刘秋润,谭慎微,刘云华,周洁平. r－干扰素对喉癌细胞系HEP－2HLA－DR抗原和增殖细胞核抗原表达的影响[J]. 中国组织化学与细胞化学杂志, 1996, 0(4) |
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| 作者姓名： | 阮清伟 刘秋润 谭慎微 刘云华 周洁平 |
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| 作者单位： | 同济医科大学附属同济医院耳鼻喉科，上海市长宁区中心医院耳鼻喉科 |
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| 摘 要： | 本实验用人重组ｒ－干扰素（ｒｈｕ－ＩＦＮ）作用ＨＥＰ－２细胞后ＨＬＡ－ＤＲ抗原和增殖细胞核抗原（ＰＣＮＡ）表达的检测来探讨ｒ－干扰素对ＨＥＰ－２细胞ＨＬＡ－ＤＲ抗原表达诱导作用及体外抗增殖活性。用单克隆抗体ＣＲ３／４３（抗ＨＬＡ－ＤＲ）和Ｋｉ－６７（抗ＰＣＮＡ）。以链霉素一生物素技术（ＬＳＡＢ）检测ＨＥＰ－２细胞ＨＬＡ－ＤＲ抗原和ＰＣＮＡ表达，结果显示：ｒ－ＩＥＮ诱导ＨＬＡ－ＤＲ抗原和抑制ＰＣＮＡ表达其强弱与ｒ－ＩＦＮ剂量有关。资料提示：ｒ－ＩＦＮ不仅对ＨＥＰ－２细胞有细胞毒作用，同时能调节其细胞膜特性，因而在喉癌的治疗中是有效的。
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| 关 键 词： | 喉癌细胞系，r－干扰素，免疫细胞化学 |
EFFECTS OF IFN-r ON HLA-DR ANTIGEN AND PROLIFERATIVE CELLULAR NUCLEUS ANTIGEN (PCNA) EXPRESSION OF HUMAN LARYNGED CANCER CELL LINE (HEP-2) |
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| Ruan Qingwei , Liu Qinrun, Tan Shenwei, Liu Yuanhua, Zhou Jieping. EFFECTS OF IFN-r ON HLA-DR ANTIGEN AND PROLIFERATIVE CELLULAR NUCLEUS ANTIGEN (PCNA) EXPRESSION OF HUMAN LARYNGED CANCER CELL LINE (HEP-2)[J]. Chinese Journal of Histochemistry and Cytochemistry, 1996, 0(4) |
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| Authors: | Ruan Qingwei Liu Qinrun Tan Shenwei Liu Yuanhua Zhou Jieping |
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| Abstract: | The effects of IFN-r on HLA-DR and proliferative cellular nucleus antigen expression ofHEP-2 cell line were investigated using LSAB (labelled sptraptaridin biotin method) withmonclonal antibody CR3/43 (anti-HLA-DR) and Ki-67 (anti-PCNA). The results showedthat HLA-DR antigen which induced and PCNA expression which is inhibited by IFN-r inHEP-2 line, and were dependent on dose. The data suggest that the IFN-r may be useful inthe treatment of larryngeal carcinotna because it provides effective cytotoxicty and cell membrance mudlating properties to HEP-2. |
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| Keywords: | Laryngeal carcinoma Cell line IFN-r Immunohistochemistry |
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