Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor |
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| Authors: | Strovas Tim J Dragavon Joe M Hankins Tyler J Callis James B Burgess Lloyd W Lidstrom Mary E |
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| Institution: | Department of Bioengineering, Microscale Life Sciences Center, University of Washington, Seattle, Washington 98195-2180, USA. |
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| Abstract: | Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes. |
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