差异显示反转录PCR技术研究进展

分析一对细胞或组织在不同状态下基因表达的差异，已成为分子生物学研究领域的热点之一.近年来，用于识别差异表达基因的方法已发展起来多种.DDRT-PCR是近年来较为广泛应用的一种技术.理论上，DDRT-PCR技术比较简单，但实施起来却存在着假阳性率高，凝胶中单条cDNA带成分不均一, 所获cDNA仅代表着mRNA 3′UT区(约300 bp)以及一些低拷贝数mRNA不能有效被呈现等问题.对DDRT-PCR技术的改良也主要集中在解决这些问题方面.

Advance of DDRT-PCR

To identify and analyze differentially expressed genes between one pair of cells or tissues in different conditions is of great importance in molecular biology study. In recent years, a variety of approaches have been used to identify differentially expressed genes. DDRT-PCR is one of the most widely used approaches. In theory, DDRT-PCR technique is simple. But in practice, some limitations such as high false-positive rate, inhomogeneity of cDNA bands, short cDNA fragments and underrepresentation of low abundance mRNAs exist. Most modifications to DDRT-PCR technique mainly focus on above aspects.