Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration |
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Authors: | Yan-qing?Yuwen Zi-mei?Dong Qing-hua?Wang Xiao-juan?Sun Chang-ying?Shi Email author" target="_blank">Guang-wen?ChenEmail author |
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Institution: | (1) Henan Normal University, Xinxiang, 453007, People’s Republic of China; |
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Abstract: | It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell
types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian
regeneration, three housekeeping genes, 18S rRNA, ACTB and DjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software.
Expression levels of the Djsix-1 gene were studied in parallel with ACTB and DjEF2 both or each and 18S rRNA as reference during regeneration. The results showed that ACTB was the most stable expressed reference gene in the planarian regeneration. |
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