首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration
Authors:Yan-qing?Yuwen  Zi-mei?Dong  Qing-hua?Wang  Xiao-juan?Sun  Chang-ying?Shi  Email author" target="_blank">Guang-wen?ChenEmail author
Institution:(1) Henan Normal University, Xinxiang, 453007, People’s Republic of China;
Abstract:It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian regeneration, three housekeeping genes, 18S rRNA, ACTB and DjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software. Expression levels of the Djsix-1 gene were studied in parallel with ACTB and DjEF2 both or each and 18S rRNA as reference during regeneration. The results showed that ACTB was the most stable expressed reference gene in the planarian regeneration.
Keywords:
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号