Proteomic identification of differentially expressed proteins in mature and germinated maize pollen |
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Authors: | Yunhao Zhu Pengfei Zhao Xiaolin Wu Wei Wang Monica Scali Mauro Cresti |
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Affiliation: | 1.College of Life Science,Henan Agricultural University,Zhengzhou,China;2.Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province,Zhengzhou,China;3.Dipartimento di Scienze Ambientali ‘G. Sarfatti’,Università di Siena,Siena,Italy |
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Abstract: | The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor α are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development. |
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