Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived <Emphasis Type="Italic">Brassica napus</Emphasis> embryos |
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Authors: | Denise M Barbulescu Wayne A Burton Philip A Salisbury |
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Institution: | (1) Department of Primary Industries, Biosciences Research Division, Brassica Germplasm Improvement Group, Private Bag 260, Horsham, VIC, 3401, Australia;(2) AgriProducts, Viterra Ltd, Private Bag 260, Horsham, VIC, 3401, Australia;(3) Melbourne School of Land and Environment, University of Melbourne, Melbourne, 3010, Australia |
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Abstract: | Recalcitrance to tissue culture is observed in some genotypes of Brassica napus. Several studies have confirmed that Pluronic F-68 has growth-promoting effects on numerous tissue types. This work investigated
the effect of the non-ionic surfactant Pluronic F-68 at four concentrations (0.1%, 0.25%, 0.5%, and 1% (w/v)) on the responsiveness of recalcitrant B. napus lines to tissue culture. Microspores from seven populations of B. napus were cultured on Nitsch and Nitsch medium with this compound. The embryos obtained were plated on solid B5 medium supplemented
with zeatin for shoot induction. Pluronic F-68 had a highly significant effect on the proportion of shoot regeneration (P < 0.05) in some of the recalcitrant populations. However, no strong dose–response effect was observed. The estimated probability
of a shoot occurring in the absence of Pluronic F-68 ranged from 0.04 to 0.31 depending on the genotype, while in the presence
of Pluronic F-68, it ranged from 0.07 to 0.53, respectively. |
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