(1) Department of Botany, North-Eastern hill University, 793 022 Shillong, India
Abstract:
While the ribosomal RNA like highly conserved genes are good molecular chronometers for establishing phylogenetic relationships,
they can also be useful in securing the amplification of adjoining hyper-variable regions. These regions can then be used
for developing specific PCR primers or PCR-RFL profiles to be used as molecular markers. We report here the use of ITS region
ofrrn operon ofFrankia for developing PCR-RFL profiles capable of discriminating between closely related frankiae. We have also made use of the
ITS 1 region of the nuclearrrn operon ofAlnus nepalensis (D Don) for designing a PCR primer for specific amplification of nuclear DNA of this tree.