凌霄花的化学成分与抗生育活性

对凌霄Campsisgrandiflora(Thunb .)K .Schum .的干燥花进行了化学成分研究 ,分离获得了 10个化合物 ,并且根据它们的物理化学性质及各种光谱数据鉴定了其中的 9个化合物。它们分别是 :三十一烷醇、α ,β 香树脂醇、β 谷甾醇、15 巯基 2 十五烷酮、芹菜素、胡萝卜甙、齐墩果酸、桂皮酸、acteoside。另一结构待定的化合物 ,暂定名为CG 10。除了其中的 β 谷甾醇、芹菜素和齐墩果酸外 ,其他化合物均首次从凌霄的花中获得。除化学成分的研究以外 ,我们还进一步考察了凌霄花乙醇浸膏的石油醚、乙酸乙酯、丙酮、丙酮 :甲醇 (1∶1)、甲醇 5部分的提取部位 ,以及分到的齐墩果酸、胡萝卜甙、acteoside及CG 10等 4个化合物收缩离体孕小鼠子宫肌条的活性。首次报道了凌霄花抗生育活性部位的抗生育活性 ,其中丙酮 :甲醇 (1∶1)提取部位可以极显著的增强孕小鼠的离体子宫肌条的收缩强度 (P <0 .0 0 1)。这个研究结果提示在临床上可能有一定的应用价值。

STUDIES ON THE CHEMICAL CONSTITUENTS OF THE FLOWER OF CAMPSIS GRANDIFLORA ( THUNB. ) K. SCHUM. AND ITS CONTRACEPTIVE EFFECT

Chinese drug"Ling-Xiao-Hua", the dried flower of Campsis grandiflora (Thunb.)K. Schum. wasspecified as Flos Campsis in Chinese Pharmacopoeia(2000). Hentriacontanol ( 1 ), α-, β-amyrin (2), β-sitosterol(3), oleanolic acid(4 ), apigenin ( 5 ), cinnamic acid (6), 15-mercapto-2-pentadecanone ( 7 ), daucosterol ( 8 ) andacteoside(9) were separated and identified by physiochemical and spectroscopic analyses. Besides β-sitosterol,oleanolic acid and apigenin, the other 7 compounds were the first time separated from the dried flower ofCampsis grandiflora(Thunb. )K. Schum. We also investigated the contraceptive effect of the five fractions ofpetroleum ether(60～ 90 ℃ PE), EtOAc(EA), Me2CO(AC), Me2CO: CH3OH(1: 1 AC: ME = 1: 1),andCH3OH(ME) ,and three compounds,oleanolic acid, daucosterol, and acteoside. We found that EA,AC,AC: ME(1:1) ,and ME fractions (0.02 rng/ml)all can distinctly improve the contractive effect on the isolated preg-nancy rat's uterine muscle(P＜0.01). And AC,AC: ME(1:1) fraction had the most distinctly effect on im-prove the contractive strength of the isolated pregnancy rat's uterine muscle(P ＜ 0.01).