Effect of agitation rate and impeller design on oxygen transfer coefficients in small bioreactors using surface aeration |
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Authors: | E M Sybert E L Winter T W Cadman M A Conner |
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Institution: | Engineering Research Center, University of Maryland, College Park 20742. |
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Abstract: | The effects of agitation rate and impeller type on the combined oxygen mass-transfer coefficient (kL a) in four different benchtop bioreactors have been examined. Surface oxygenation of a cell culture medium supplemented with fetal bovine serum and distilled deionized water has been studied by passing air through the bioreactor headspace at approximately one headspace volume per minute. A new ribbon-type impeller design using strips of Teflon has been shown to be superior to conventional impeller designs for oxygen transfer. |
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