Tunable Assembly of Peptide-coated Gold Nanoparticles |
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Authors: | Jennifer A Tullman William F Finney Yu-Jen Lin Sandra Whaley Bishnoi |
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Institution: | (1) Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, 3101 S. Dearborn St., Chicago, IL 60616, USA;(2) Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA |
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Abstract: | The interaction between peptides and gold surfaces has increasingly been of interest for bionanotechnology applications. To
more fully understand how to control such interactions, we have studied the optical properties of peptide-modified gold nanoparticles
as a function of peptide composition, pH of the surrounding medium, and peptide concentration. We show using localized surface
plasmon resonance, transmission electron microscopy, and surface-enhanced Raman scattering (SERS) that selected “gold-binding
peptides” (GBPs), similar to those isolated for binding to gold films using yeast display, can bind to gold nanoparticles
at a variety of pHs. Peptide modifications of nanoparticles can lead to irreversible particle aggregation when the pH of the
solution is kept below the isoelectric point (pI) of the peptide. However, at pHs above the peptide’s pI, particles remain
stable in solution, and peptides remain bound to the particles possibly through amine coordination of gold. Additionally,
we demonstrate the potential in using SERS for the direct detection of GBPs on gold-silica nanoshells, eliminating the need
for indirect labeling methods. |
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Keywords: | Peptide Gold Nanoparticles Assembly |
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