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Sandwich Embedding of Multiple Layers of Muscle Tissue Culture for Serial Frozen Sectioning
Authors:Valerie Askanas  Saiyid A Shaeiq  Dennis Adamchak
Institution:  a Institute for Muscle Disease, Inc., New York, NY
Abstract:Serial sections of fresh-frozen skeletal muscle fibers are necessary for demonstrating comparative histochemical relationships between oxidative enzymes and either myofibrillar adenosine triphosphatase or glycolytic enzymes in different fiber types (Dubowitz and Pearse 1960, Engel 1962).
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