Characterization and role of a metalloprotease induced by chitin in <Emphasis Type="Italic">Serratia</Emphasis> sp. KCK |
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Authors: | Hyun-Soo Kim Peter N Golyshin Kenneth N Timmis |
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Institution: | (1) Department of Environmental Microbiology, The Helmholtz Center for Infection Research, Braunschweig, Germany;(2) Institute for Microbiology, Technical University of Braunschweig, Biozentrum, Braunschweig, Germany |
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Abstract: | A metalloprotease induced by chitin in a new chitinolytic bacterium Serratia sp. Strain KCK was purified and characterized. Compared with other Serratia enzymes, it exhibited a rather broad pH activity
range (pH 5.0–8.0), and thermostability. The cognate ORF, mpr, was cloned and expressed. Its deduced amino acid sequence showed high similarity to those of bacterial zinc-binding metalloproteases
and a well-conserved serralysin family motif. Pretreatment of chitin with the Mpr protein promoted chitin degradation by chitinase
A, which suggests that Mpr participates in, and facilitates, chitin degradation by this microorganism. |
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Keywords: | Metalloprotease Chitin Serratia Chitinase A |
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