小鼠膜联蛋白Ⅱ基因剔除重组子的构建及小鼠无膜联蛋白Ⅱ细胞系的建立

为了便于对膜联蛋白II(annexinII)的进一步研究以及今后进一步发展膜联蛋白II-/-动物模型,构建了膜联蛋白II基因封闭重组子(pPNT/annexinII/LacZ),筛选了细胞(L5178Y)克隆,并获得了膜联蛋白II-/-稳定细胞克隆(D4,E2)。所获膜联蛋白II-/-L5178Y克隆有待于以PCR做进一步鉴定。

Construction of Mouse Annexin II Gene Knockout Recombinant and Generation of Mouse Annexin II-/- Cell Line

Annexins are Ca2 and phospholipids binding proteins forming an evolutionary conservedmultigene family with members of the family being expressed throughout animal and plant kingdoms.Some mem-bers of the family participate in the regulation of membrane organization,membrane traffic,and the regulation of ion(Ca2 ) currents across membranes or Ca2 concentration within cells.Recently,several cellular or animal annexinknockout models as well as dominant-negative mutants of the family have been established.As an important memberof annexin family,annexin II has been proved to be involved with and play some important roles in the developmentof some human diseases.The paper reports that a gene knockout recombinant(pPNT/annexinII/LacZ) was con-structed for blocking the mouse annexin II expression,and two cell clones(D4 and E2) were screened out as theannexin II-/-L5178Y cell clones that will be confirmed further more using PCR.The annexin II knockout recombi-nant and the annexin II-/-cell line can be used to generate annexin II null mice and further researches on annexin IIin future.