Composition of photosynthetic pigments in thylakoid membrane vesicles from spinach |
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Authors: | René K. Juhler Eva Andreasson Shi-Gui Yu Per-Åke Albertsson |
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Affiliation: | (1) Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark;(2) Department of Biochemistry, Chemical Center, University of Lund, P.O. Box 124, S-221 00 Lund, Sweden |
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Abstract: | Thylakoid membranes from spinach were fragmented mechanically and separated into vesicles originating from grana and stroma-exposed lamellae (Andreasson et al. (1988) Biochim Biophys Acta 936: 339–350). The grana vesicles were further fragmented and separated into smaller vesicles originating from different parts of the grana (Svensson and Albertsson (1989) Photosynth Res 20: 249–259). All vesicles so obtained were analyzed with respect to chlorophyll and carotenoid composition by reverse phase HPLC. For all fractions the following relations (mole/mole) were found: 1 carotenoid per 4 chlorophyll (a+b), 2 lutein per 5 chlorophyll b and 5 violaxanthin per 100 chlorophyll (a + b). The contents of lutein and neoxanthin were each linearly related to chlorophyll b and -carotene was linearly related to chlorophyll a. |
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Keywords: | carotenoids chlorophylls HPLC phase partition xanthophylls |
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