3种苦参生物碱对小鼠的毒性作用研究

以小鼠为试验对象,对3种苦参生物碱(氧化苦参碱、槐定碱、苦参碱)的毒性作用进行了系统的分析.结果显示:(1)混合后的苦参生物碱毒性增加,其中苦参碱+槐定碱的增效作用最强,协同毒力指数(c.f)值达59.1,氧化苦参碱+苦参碱混合给药,c.f值为37.3,为增效作用;氧化苦参碱+槐定碱混合给药,c.f值为13.6,为相加作用.(2)血常规检测结果显示,3种苦参生物碱处理组小鼠的白细胞计数(WBC)、血小板总数(PLT)、中粒细胞(GR)较之对照组显著增高;血液生化指标检测结果显示,槐定碱和氧化苦参碱各处理组小鼠的尿素氮(BUN)、肌酐(Cr)水平均极显著高于对照组(P＜0.01),苦参碱各处理组小鼠的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基酸转移酶(AST)水平比对照组极显著升高(P＜0.01);脏器系数的统计结果显示:槐定碱、氧化苦参碱各处理组小鼠的肾脏系数、心脏系数以及肝脏系数均极显著高于对照组(P＜0.01),苦参碱处理组小鼠的肾脏系数、心脏系数及肺脏系数显著地高于对照组(P＜0.01、P＜0.05、P＜0.05).研究表明,3种苦参生物碱对小鼠肝脏、肾脏有影响,初步确定它们作用的靶器官是肝脏和肾脏.

Toxicity of Three Sophora flavescens Ait Alkaloids to Mice

The toxicity of three Sophora flavescens Ait alkaloids(Oxymatrine,sophoridine,matrine) to mice were systematically researched in this paper.Results are as below:the toxicity of Sophora flavescens Ait alkaloids which acted on mice were increased after mixture.In matrine and sophoridine group,c.f value was 59.1.In oxymatrine and matrine group,c.f value was 37.3,showing the synergy effects;In oxymatrine and sophoridine group,c.f value was 13.6 which indicated that had additive effect.The results of blood routi...