Induction of l-lysine ɛ-aminotransferase by l-lysine in Streptomyces clavuligerus, producer of cephalosporins

Abstract Several alcohols were examined as substrates for the polyhydroxyalkanoate synthesis by Paracoccus denitrificans. The bacterium synthesized a homopolyester of poly(3-hydroxybutyrate) from ethanol. When n -pentanol was used as growth substrate, homopolyester poly(3-hydroxyvalerate) was synthesized, whereas copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulated during bacterial growth on n -propanol. When alcohols were automatically fed as growth substrates, ethanol, n -propanol, and n -pentanol gave higher polyester content. Although poly(3-hydroxybutyrate) was synthesized from methanol or n -butanol, its content was very low. Under nitrogen-deficient conditions, polyester;content in cells increased, especially with ethanol, n -propanol, and n -pentanol. Using a mixture of two alcohols P. denitrificans could synthesize polyesters with varying relative ratios of 3-hydroxybutyrate to 3-hydroxyvalerate.