| 叶绿体SSR标记：柑橘体细胞杂种胞质遗传分析的一种新方法 |
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| 引用本文： | 程运江,郭文武,邓秀新.叶绿体SSR标记：柑橘体细胞杂种胞质遗传分析的一种新方法[J].Acta Botanica Sinica,2003,45(8):906-909. |
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| 作者姓名： | 程运江 郭文武 邓秀新 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室，华中农业大学作物遗传改良国家重点实验室，华中农业大学作物遗传改良国家重点实验室 武汉 430070，武汉 430070，武汉 430070 |
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| 基金项目： | 国家自然科学基金(30123001)，国家高技术研究发展计划“863”项目(2001AA241141)，国际基金(IFS)，国际植物种质资源委员会(IPGRI) |
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| 摘 要： | 从水稻(Oryza sativa L．)、烟草(Nicotiana tabacum L．)和黑松(Pinus thunbergii Parl．)等植物的22对叶绿体SSR引物中筛选出5对能用于柑橘叶绿体SSR分析的引物，应用这5对引物对9个组合的柑橘体细胞杂种的叶绿体遗传进行了分析。结果表明：这些组合再生的杂种中叶绿体都呈现随机分离，该现象与以前报道的RFLP分析结果一致，而且其可靠性已被CAPS分析所证实。表明柑橘叶绿体SSR同RFLP及CAPS一样可靠，并且更简单高效、易于操作，特别适合对柑橘等植物体细胞杂种进行早期胞质遗传组成分析。
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| 关 键 词： | 柑橘 体细胞杂种 微卫星序列 简单序列重复 叶绿体基因组 胞质遗传 体细胞杂种 叶绿体SSR标记 |
cpSSR: a New Tool to Analyze Chloroplast Genome of Citrus Somatic Hybrids |
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| Authors: | CHENG Yun-Jiang GUO Wen-Wu DENG Xiu-Xin |
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| Abstract: | Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Part), rice (Oryza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses. |
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| Keywords: | Citrus somatic hybrids microsatellite simple sequence repeat (SSR) chloroplast genome |
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