Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.) |
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Authors: | Shashi Kant Singh Manoj K. Rai Pooja Asthana L. Sahoo |
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Affiliation: | (1) Center for Energy, Indian Institute of Technology, Guwahati, 781039, Assam, India;(2) Laboratory of Morphogenesis, Department of Botany, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India;(3) Present address: Department of Life Science, Institute of Plant Biology, National Taiwan University, Taipei, Taiwan |
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Abstract: | Nodal segments obtained from in vitro proliferated shoots of Eclipta alba (L.) Hassk, were encapsulated in calcium alginate beads for large-scale clonal propagation, short-term conservation and germplasm exchange and distribution. The best gel complexation was achieved using 3% sodium alginate and 100 mM CaCl2·2H2O. Maximum percent response (100%) for conversion of encapsulated nodal segments into plantlets was obtained on 0.7% agar-solidified full-strength MS medium containing 0.88 μM BAP. Encapsulated nodal segments could be stored at low temperature (4°C) up to 60 days with a survival frequency of 51.2%. The well-developed plantlets regenerated from encapsulated nodal segments were hardened-off successfully with 90% survival frequency. |
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