Designing a whole-cell biotransformation system in Escherichia coli using cytochrome P450 from Streptomyces peucetius |
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| Authors: | Pramod Shrestha Tae-Jin Oh Jae Kyung Sohng |
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| Affiliation: | (1) Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), SunMoon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, Republic of Korea |
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| Abstract: | A biotransformation system was designed to co-express CYP107P3 (CSP4), cytochrome P450, from Streptomyces peuceticus, along with CamA (putidaredoxin reductase) and CamB (putidaredoxin) from Pseudomonas putida, the necessary reducing equivalents, in a class I type electron-transfer system in E. coli BL21 (DE3). This was carried out using two plasmids with different selection markers and compatible origins of replication. The study results showed that this biotransformation system was able to mediate the O-dealkylation of 7-ethoxycumarin. |
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| Keywords: | Co-expression Cytochrome P450 Streptomyces peuceticus Whole-cell biotransformation |
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