| 海藻酸钠包埋法制备固定化菠萝蛋白酶 |
| |
| 引用本文： | 张琛,张宽朝,章琛,缪伟. 海藻酸钠包埋法制备固定化菠萝蛋白酶[J]. 生物加工过程, 2009, 7(6): 31-34. DOI: 10.3969/j.issn.1762-3678.2009.06.006 |
| |
| 作者姓名： | 张琛 张宽朝 章琛 缪伟 |
| |
| 作者单位： | 安徽农业大学,生命科学学院,合肥,230036 |
| |
| 基金项目： | 国家自然科学基金资助项目，安徽农业大学校综合性实验项目 |
| |
| 摘 要： | 以海藻酸钠为载体，包埋法固定菠萝蛋白酶，对固定化奈件进行优化，同时探讨固定化菠萝蛋白酶的部分酶学性能。结果表明：固定化菠萝蛋白酶的质量受海藻酸钠质量分数、固定化酶量、固定化时间以及CaCl2质量分数的影响，其最佳固定化条件为：海藻酸钠质量分数1．0％，CaCl2质量分数3％，固定化酶液量与海藻酸钠体积之比1：2，固定化时间60min，在此条件下，制备的固定化菠萝蛋白酶的比活力为211．8U／g（湿质量载体），由此制得的固定化酶的最适pH为7．6，与游离酶相比，升高了0．8个pH单位，同时显示固定化菠萝蛋白酶能耐受较高的碱性环境，固定化酶最适温度与游离酶相同，均为50℃，固定化酶在较高温度范围内，仍能保持较高的相对活力。
|
| 关 键 词： | 海藻酸钠 固定化 菠萝蛋白酶 |
Immobilization of bromelain by embedding in sodium alginate |
| |
| ZHANG Chen,ZHANG Kuan-chao,ZHANG Chen,MIAO Wei. Immobilization of bromelain by embedding in sodium alginate[J]. Chinese Journal of Bioprocess Engineering, 2009, 7(6): 31-34. DOI: 10.3969/j.issn.1762-3678.2009.06.006 |
| |
| Authors: | ZHANG Chen ZHANG Kuan-chao ZHANG Chen MIAO Wei |
| |
| Affiliation: | ( College of Life Science, Anhui Agricultural University, Hefei 230036, China) |
| |
| Abstract: | Sodium alginate was used as a carrier for the immobilization of bromelain. The optimum operation conditions were as follows: the concentration of sodium alginate and CaCl_2 solution were 1.0% and 3.0%, respectively; the ratio of enzyme solution to sodium alginate was 1∶2; the immobilization time was 60 min.Under these conditions, the optimum pH of the immobilized enzyme was shifted form 6.8 to 7.6, and the optimum reaction temperature was 50 ℃. |
| |
| Keywords: | sodium alginate immobilization bromelain |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
