Monoclonal antibodies to cell surface components of zoospores and cysts of the fungusPythium aphanidermatum reveal species-specific antigens |
| |
| Institution: | 1. School of Biological Sciences, University of Birmingham, P.O. Box 363, Birmingham, B15 2TT, United Kingdom;2. Institute of Horticultural Research, Wellesbourne, Warwick, CV35 9EF, United Kingdom;1. Department of Prosthodontics, Shanghai Ninth People''s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China;2. National Clinical Research Center for Oral Diseases, Shanghai, 200011, China;3. Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai, 200011, China;4. Fengcheng Hospital of Fengxian District, Shanghai, 201411, China;1. Institute of Biology, Laboratory of Soil Biology, University of Neuchâtel, CH-2009 Neuchâtel, Switzerland;2. Instituto de Botánica Spegazzini, Facultad de Cs. Naturales y Museo, Universidad Nacional de La Plata, 53 N° 477, 1900 La Plata, Buenos Aires, Argentina;3. CADIC, CONICET. Lab. Ecología Terrestre, Bernardo Houssay 200, Ushuaia, Tierra del Fuego, Argentina;4. Forest Research Institute, Braci Lesnej St., Sekocin Stary, 05-090 Raszyn, Poland |
| |
| Abstract: | A panel of monoclonal antibodies (MAbs) designated PA1 to PA8 has been raised against cell surface components of zoospores and cysts of the pathogenic fungusPythium aphanidermatum. The antibodies were selected on the basis of binding assays using indirect immunofluorescence. Four binding patterns were observed: PA1 labeled the entire zoospore surface including both flagella, PA2 binding was restricted to the anterior flagellum, PA3–PA6 bound to the adhesive cell coat secreted by zoospores during encystment, and PA7 and PA8 labeled zoospores and the cyst cell wall. Electron microscopic immunogold labeling of zoospores showed that PA2 bound to the mastigonemes on the anterior flagellum. The MAbs were tested for binding to zoospores and cysts of several isolates ofP. aphanidermatum, and to zoospores and cysts of several species ofPythium, Phystophthora, Aphanomyces, andSaprolegnia. The results showed that the antigens recognized by MAbs PA1–PA6 were restricted toP. aphanidermatum, whereas those recognized by PA7 and PA8 occurred on all species tested. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
