Improved EBV shuttle vectors |
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Institution: | 1. Leukocyte Biology Section, National Heart & Lung Institute, Imperial College London, London, UK;4. Division of Pharmacology–Pharmacotechnology, Biomedical Research Foundation of the Academy of Athens, Athens, Greece;5. DNA Vector Research, German Cancer Research Centre (DKFZ), Heidelberg, Germany |
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Abstract: | Shuttle vectors based on Epstein-Barr virus (EBV) replicate autonomously in the nuclei of human cells. These vectors represent reasonable models for chromosomes, have low background mutation frequencies, and have been useful for studying induced mutation in human cells. Two improvements in the EBV vector system are discussed. Attempts are described to increase vector copy number per cell by using a limited period of replication driven by the simian virus 40 (SV40) origin of replication. Isolation of human sequences that can replace the viral origin of replication in providing for autonomous replication of the vectors is also described. These improvements are leading toward shuttle vectors that are more efficient and more closely resemble authentic chromosomes. |
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