Purification of a melanin biosynthetic enzyme converting scytalone to 1,3,8-trihydroxynaphthalene from Cochliobolus miyabeanus |
| |
| Institution: | 1. Department of Biology, Faculty of Science, Thaksin University, Phatthalung Campus, Phatthalung, Thailand;2. National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang, Pathum Thani 12120, Thailand;3. Department of Microbiology and Natural Product Research Center of Excellence, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla, Thailand;4. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla, Thailand;1. State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100101, China;3. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;4. Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin 300071, China;1. Innovation Center of Chinese Medicine, China State Institute of Pharmaceutical Industry, No. 285 Gebaini Road, Pudong New District, 201203, Shanghai, PR China;2. College of Medicine, Zhengzhou University, No. 100 Kexue Road, Zhongyuan District, 450001, Zhengzhou, PR China;3. Medical School, Huanghe Science & Technology College, No. 94 Hanghaizhong Road, Zhongyuan District, 450006, Zhengzhou, Henan, PR China;1. Faculty of Agriculture, Kagawa University, Miki, Japan;2. Faculty of Science and Technology, Keio University, Yokohama, Japan;3. The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan;1. Department of Oncology, PLA General Hospital, PLA School of Medicine, Beijing, China;2. National Clinical Research Center for Normal Aging and Geriatric & The Key Lab of Normal Aging and Geriatric, Institute of Geriatric, PLA General Hospital, Beijing, China;1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, Guangdong, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China |
| |
| Abstract: | A melanin biosynthetic enzyme, scytalone dehydratase, which converts scytalone to 1,3,8-trihydroxynaphthalene was purified from mycelia of Cochliobolus miyabeanus. Enzymatic reactions were carried out under anaerobic conditions to prevent unfavorable oxidative reactions. This enzyme had an optimum pH near 8.2 and mol w 23,000 Da. The enzymatic reaction did not require NADPH as a cofactor and was not inhibited by the melanin biosynthesis inhibitors, tricyclazole, PP 389, pyroquilon, PCBA, and phthalide |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
