Woronin bodies fromPenicillium chrysogenum: Isolation and characterization by analytical subcellular fractionation |
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| Institution: | 1. Instituto de Investigación de la Cadena Láctea (INTA-CONICET), Estación Experimental Agropecuaria Rafaela, Ruta 34 Km 227, Rafaela, Santa Fe, Argentina;2. Laboratorio de Inmunología Experimental, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, S3000ZAA, Santa Fe, Argentina;1. Mental Health and Behavioral Sciences, VA New Jersey Healthcare System, 151 Knollcroft Road, Lyons, NJ 07939, USA;2. Department of Psychiatry, Robert Wood Johnson Medical School, The State University of New Jersey, Piscataway, NJ, USA;3. Research and Development Service, VA New Jersey Healthcare System, East Orange, NJ, USA;4. Department of Pharmacology, Physiology, and Neuroscience, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, USA;5. Department of Psychology, William Paterson University, Wayne, NJ, USA;6. War Related Illness and Injury Study Center, VA New Jersey Healthcare System, East Orange, NJ, USA;7. Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York State Psychiatric Institute, New York, NY, USA;1. IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy;2. Università degli Studi di Milano, Department of Pathophysiology and Transplantation, Milan, Italy;3. QIMR Berghofer Medical Research Institute, Brisbane, Australia;4. Queen Silvia Children''s Hospital, Gothenburg, Sweden;5. Ospedale Pediatrico Bambino Gesù, Rome, Italy;6. City Children''s Hospital N1, Yaroslavl, Russia;7. Starship Children''s Health, Auckland, New Zealand;8. Federal State Budgetary Scientific Institution «Research Centre for Medical Genetics», Moscow, Russia;9. Queensland Children''s Hospital, Brisbane, Australia;10. Karolinska University Hospital, Stockholm, Sweden;11. Royal Children''s Hospital, Melbourne, Australia;12. Sydney Children''s Hospital & School of Women''s and Children''s Health, Medicine, University of New South Wales, Sydney, Australia;13. ASST Spedali Civili Brescia, Brescia, Italy;1. Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Sydney, Australia;2. St. Vincent''s Clinical School, University of New South Wales, Sydney, Australia;3. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia |
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| Abstract: | Differential centrifugation of whole homogenates ofPenicillium chrysogenum, disrupted by a modified Ballotini bead method, resulted in the enrichment of Woronin bodies between 800g (5 minutes) and 6000g (10 minutes). Isolated Woronin bodies are membrane-bounded, electron-opaque, approximately spherical organelles, 0.11 to 0.29 μm in diameter. Woronin bodies have a buoyant density (ϱ) of 1.21 g cm−3 and S20,w values of 6300 to 37,600 in sucrose gradients. Analytical subcellular fractionation of whole homogenates in a zonal rotor showed that Woronin bodies did not cosediment with marker enzymes for lysosomes (acid phosphatase), peroxisomes (catalase), mitochondria (cytochrome c oxidase), or endoplasmic reticulum (NADPH cytochrome c reductase). |
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