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Wood hydrolyzate treatments for improved fermentation of wood sugars to 2,3-butanediol
Institution:1. Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea;2. Department of Chemical and Biological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 02841, Republic of Korea;3. Department of Environmental Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea;4. Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 04107, Republic of Korea;5. Department of Biotechnology, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 02841, Republic of Korea;1. Department of Sustainable Bioproducts, Mississippi State University, Box 9820, Mississippi State, MS 39762, United States;2. Cellulose & Paper Department, National Research Center, Dokki, Cairo, Egypt
Abstract:Acid-hydrolyzed hardwood contains compounds inhibitory to microorganisms that convert wood sugars to fermentation products such as fuels and chemicals. Several methods of treating acid-hydrolyzed hardwood (hydrolyzate) to reduce the levels of potential microbial inhibitors (acetate, furfural, sulfate, and phenolics) were evaluated. The methods evaluated were precipitation with calcium hydroxide, extraction with organic solvents, treatment with ion-exchange resins, adsorption resins, and activated charcoal. Treatment of the hydrolyzate with an anion exchange resin (Amberlite IRA-400) was the most effective method for removing potential inhibitors. Non-treated hydrolyzate adjusted to pH 6 inhibited growth of a 2,3-butanediol-producing culture of Klebsiella pneumoniae. However, hydrolyzate treated with Amberlite IRA-400 was not inhibitory and resulted in yields of 2,3-butanediol that were greater than 90% of theoretical.
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