Selective isolation of bacteria from soil with hydrophobic materials |
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Authors: | Oku Shota Nishiyama Masaya Takao Yuji |
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Institution: | (1) Faculty of Environmental Studies, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan; |
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Abstract: | Bacterial strains having a hydrophobic cell surface have often been considered as degraders of hydrophobic organic pollutants
in soil. In this study, bacterial strains were isolated using hydrophobic materials from 12 soil samples, and their cell surface
hydrophobicity was determined by evaluating their adherence to n-hexane. Bacterial strains isolated using polytetrafluoroethylene (PTFE) membrane were more hydrophobic on an average than
those isolated with styrene–divinylbenzene (DVB) particles or octadecylsilyl silica gel (ODS) particles. Strains closely related
to Burkholderia cepacia could be selectively isolated using the PTFE membrane; those closely related to Ralstonia pickettii, using ODS and DVB particles; and those closely related to B. fungorum, using DVB. These results indicate that bacterial strains having a hydrophobic cell surface or within certain phyla can be
selectively isolated from soils using hydrophobic materials, and that this isolation method would be useful for collecting
candidates for bioremediation of hydrophobic pollutants. |
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Keywords: | |
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