囊泡膜谷氨酸转运体阳性纤维在大鼠三叉神经运动核内的分布

目的观察I、Ⅱ型囊泡膜谷氨酸转运体阳性纤维在大鼠三叉神经运动核内的分布。方法首先采用免疫荧光三重标记I、Ⅱ型囊泡膜谷氨酸转运体和神经元核蛋白以观察I、Ⅱ型囊泡膜谷氨酸转运体阳性纤维在大鼠三叉神经运动核内的分布；接着注射四甲基罗达明人下颌舌骨肌神经逆行标记三叉神经运动核开口神经元，再采用免疫荧光双重标记I型囊泡膜谷氨酸转运体和神经元核蛋白以观察I、Ⅱ型囊泡膜谷氨酸转运体阳性纤维在大鼠三叉神经运动核开口神经元区和闭口神经元区内的分布差异。结果I型囊泡膜谷氨酸转运体阳性纤维仅在三叉神经运动核背外侧部分布，而Ⅱ型囊泡膜谷氨酸转运体阳性纤维在整个三叉神经运动核内分布；开口神经元区未观察到I型囊泡膜谷氨酸转运体阳性终末。结论闭口神经元接受I、Ⅱ型囊泡膜谷氨酸转运体阳性纤维支配，开口神经元仅仅接受Ⅱ型囊泡膜谷氨酸转运体阳性纤维支配。

Expression of vesicular glutamate transporters in the rat trigeminal motor nucleus

Objective Distribution of vesicular glutamate transporters, VGLUT1 and VGLUT2, im- munoreactivity was studied in the trigeminal motor nucleus of rats. Methods Firstly, immunofluorescence staining for VGLUT1 , VGLUT2 and neuronal nuclear protein was done in the rat; Sechondly, jaw-open- ing neurons were retrogradely labeled by injecting tetramethyl rhodamine into the rat mylohyoideus nerve, and then dual fluorescent immunostained for VGluT1 and neuronal nuclear protein. Results VGLUT1 im- munoreactivity was detected in the dorsolateral divisions of the trigeminal motor nucleus, not in the ven- tromedial divisions; whereas VGLUT2 immunoreactivity were localized throughout the trigeminal motor nucleus. The divisions without VGLUT1 immunoreactivity was right the ventromedial divisions of the tri- geminal motor nucleus containing motoneurons innervating the jaw-opening muscles. Conclusion These re- suits show that the iaw-closing motoneurons might be innervated by both VGLUTl-immunoreactive pro- files and VGLUT2-immunoreactive profiles, while the jaw-opening motoneurons might be innervated only by VGLUT2-immunoreactive profiles.