结直肠癌中Claudinl、ZEB-1和E-cadherin的表达及意义

目的探讨Claudinl、ZEB-1和E-cadherin在结直肠癌中的表达及三者之间的关系与临床病理的联系。方法免疫组织化学技术检测51例结直肠癌及癌旁组织中Claudinl、ZEB-1和E—cadherin蛋白的表达情况。结果在结直肠癌组织中Claudinl、ZEB-1、E—cadherin的表达率分别为68．6％、23．5％、56．9％，癌旁组织分别为37．3％、0、98．0％。三种蛋白在结直肠癌组织及癌旁组织中的表达差异均具有显著性（P〈O．01）。Claudinl及ZEB-1的表达率在III_IV期组（87．5％、41．7％）高于I-II期组（51．9％、7．4％；P〈0．01、P〈0．01），有淋巴结转移组（90．9％、45．5％）高于无淋巴结转移组（51．7％、6，9％；P〈0．01、P〈0．01），ZEB-1表达率在高级别组（42．1％）高于低级别组（12．5％；P〈0．05）。E—cadherin的表达率在I—II期组（74．1％）高于Ⅲ—IV期组（37．5％；P〈O．05），无淋巴结转移组（72．4％）高于有淋巴结转移组（36．4％；P〈0．05）。Claudinl、ZEB-1与E-cadherin的表达均呈负相关（P〈0．05），Claudinl与ZEB-1的表达呈正相关（P〈0．05）。结论Claudinl、ZEB-1和E-cadherin在结直肠癌发展，浸润和转移中起着重要作用，三者可能存在协同作用。

Expression and Significance of Claudin1 ,ZEB-1 and E-cadherin in Colorectal Carcinoma

Objective To study the expression of Claudinl,ZEB-1 and E-cadherin in colorectal cancer, and to investigate the relationship between them and clinicopathologic parameters. Methods Immunohistochemical staining was conducted to detect the expression of Claudinl,ZEB-1 and E-eadherin proteins in 51 samples of colorectal tumor tissues and 51 samples of normal colorectal mucosa. Results The positive rates of Claudinl,ZEB-1 and E-cadherin were 68.6% ,23.5%,56.9% in colorectal tumor and 37.3~//00,0%,98.0% in normal colorectal mucosa, with a significant difference between the two groups （P〈0. 01）. The expression rates of Claudinl and ZEB-1 in III-IV stages （87.5%,41.7%） was higher than I-II stage （51.9%, 7.4 % ;P〈0.01,P〈0. 01）, as in the cases with lymph node metastasis （90. 9 %, 45.5 %） than in those without metastasis （51.7%,6,9%~P~0.01,P%0.01）. The expression rate of ZEB-1 in high grade （42.1%） was also higher than in low grade （12.5% ,P〈0. 05）. The expression rate of E-cadherin in I-II stages （74.1%） was higher than in III-IV stages （37.5% ,P%0. 05） ,and higher in the cases with no lymph node metasta- sis （72.4%）than in those with metastasis （36.40% ,P〈0.05）. Positive correlations were observed between Claudinl and ZEB-1 in CRC tissues（P〈0.05）. The expression of E-cadherin was negatively correlated with that of Claudinl and ZEB-1 （P〈0.05）. Conclusion Expressions of Claudinl, ZEB-1 and E-cadherin may be involved in the progression,invasion and metastasis of CRC. Interactions between Claudinl,ZEB-1 and E-cadherin may promote CRC progression.