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Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene
Authors:Lan Li  Wei Shen  Lingjiang Min  Qingyu Pan  Yujiang Sun  Jixian Deng  Qingjie Pan
Institution:1. College of Animal Science and Technology, Qingdao Agriculture University, Qingdao, 266109, China
2. Institute of Biotechnology, Academy of Military Medical Science, Beijing, 100071, China
3. Yantai Fushan Advanced Vocation School, Yantai, 265500, China
4. Institute of Dongying Agricultural Sciences, Dongying, 257000, China
Abstract:Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropri-ate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to pro-duce mammary gland bioreactors.We established an effi-cient gene transfer and nuclear transfer approach in goat somatic cells.Gene targeting vector pGBC2LF was con-structed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene.Goat fetal fibroblasts were transfected with lin-earized pGBC2LF and 14 cell lines were positive accord-ing to PCR and Southern blot.The transgenic cells were used as donor cells of nuclear transfer and some of recon-structed embryos could develop into blastocyst in vitro.
Keywords:human lactoferrin  gene transfer  nuclear transfer  goat  mammary gland bioreaetor
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