| 小鼠胚胎干细胞（ES细胞）建系和维持过程中的问题及对策 |
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| 引用本文： | 孟国良,汤富酬,滕路,尚克刚. 小鼠胚胎干细胞（ES细胞）建系和维持过程中的问题及对策[J]. 遗传, 2001, 23(3): 292-294 |
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| 作者姓名： | 孟国良 汤富酬 滕路 尚克刚 |
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| 作者单位： | 北京大学生命科学学院，北京 100871College of Life Science , Peking University，Beijing 100871，China |
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| 基金项目： | 国家自然科学基金重点项目（39730250）资助 |
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| 摘 要： | 总结了十多年来我们从事ES细胞建系和培养工作的经验和教训，提出了研究工作中存在的问题以及解决这些问题的方法和对策，并对一些常规性的操作步骤进行了改进。同时，对某些尚不明确的问题进行了讨论，提出了我们的看法和建议。研究工作表明，我们采取的对策和方法是可行、有效的。Abstract：Based on manipulating Embryonic Stem cells for more than ten years, we proposed our methods to solve the problems in establishing an d maintaining ES cell lines, especially the improvement of routine operational a pproach.We also discussed some ambiguous problems and gave corresponding opinion and suggestion which was proved to be feasible and effective in our own research.
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| 关 键 词： | 正常二倍体核型 Keywords ES cell 内细胞团（ICM） 多方向分化潜能 ES细胞 |
| 文章编号： | 0253-9772(2001)03-0292-03 |
| 修稿时间： | 2000-10-17 |
The Problems and SolutionMethods in Establishing and Maintaining ES Cell Lines |
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| MENG Guo-liang,TANG Fu-chou,TENG Lu,SHANG Ke-gang. The Problems and SolutionMethods in Establishing and Maintaining ES Cell Lines[J]. Hereditas, 2001, 23(3): 292-294 |
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| Authors: | MENG Guo-liang TANG Fu-chou TENG Lu SHANG Ke-gang |
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| Abstract: | Based on manipulating Embryonic Stem cells for more than ten years, we proposed our methods to solve the problems in establishing and maintaining ES cell lines, especially the improvement of routine operational approach.We also discussed some ambiguous problems and gave corresponding opinion and suggestion which was proved to be feasible and effective in our own research. |
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| Keywords: | ES cell Inner cell mass pluripotency normal diploid karyotype |
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