5-ht2a Receptors in rat Sciatic Nerves and Schwann Cell Cultures |
| |
| Authors: | Guido M. Gaietta Elizabeth J. Yoder Tom Deerinck Karen Kinder Abraham Hanono Areum Han Chun Wu Mark H. Ellisman |
| |
| Affiliation: | 1. National Center for Microscopy and Imaging Research at San Diego, Department of Neurosciences, University of California, San Diego, La Jolla, California, USA
2. Molecular and Cellular Biology, PharMingen, San Diego, California, USA
|
| |
| Abstract: | Pharmacological approaches and optical recordings have shown that Schwann cells of a myelinating phenotype are activated by 5-HT upon its interaction with the 5-HT2A receptor (5-HT2AR). In order to further characterize the expression and distribution of this receptor in Schwann cells, we examined rat sciatic nerve and cultured rat Schwann cells using probes specific to 5-HT2AR protein mRNA. We also examined the endogenous sources of 5-HT in rat sciatic nerve by employing both histochemical stains and an antibody that specifically recognizes 5-HT. Rat Schwann cells of a myelinating phenotype contained both 5-HT2AR protein and mRNA. In the healthy adult rat sciatic nerve, 5-HT2ARs were evenly distributed along the outermost portion of the Schwann cell plasma membrane and within the cytoplasm. The most prominent source of 5-HT was within granules of the endoneurial mast cells, closely juxtaposed to Schwann cells within myelinating sciatic nerves. These results support the hypothesis that the 5-HT receptors expressed by rat Schwann cells in vivo are activated by the release of 5-HT from neighboring mast cells. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
