Isolation and continuous cultivation of Trypanosoma theileri in media containing tissue culture fluids

Tissue culture fluids, supplemented with newborn calf serum and bovine blood or with broth, newborn calf serum and blood or blood products were, respectively, suitable for the isolation and continuous cultivation of Trypanosoma theileri at 37 °C. Culture of the parasite at 37 °C in large volumes was accomplished in a tissue culture fluid-broth-blood coagulum medium. The organism was unaffected by high concentrations of penicillin or dihydrostreptomycin but was susceptible to nystatin. Culture forms of T. theileri remained viable for at least 50 days at −70 °C. An infection rate of 88% was observed in 17 cattle examined.