Induction of streptomycin resistance in the wild tomato Lycopersicon peruvianum |
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Authors: | C E Jansen E A M Snel M J E Akerboom H J J Nijkamp and J Hille |
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Institution: | (1) Department of Genetics, Free University, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands |
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Abstract: | Summary A protoplast mutagenesis and cell selection system was used for the isolation of streptomycin resistant Lycopersicon peruvianum colonies. Protoplasts were treated with the mutagen N-nitroso-methylurea and could be regenerated into fertile plants, carrying the streptomycin resistant character. Several classes of streptomycin resistance could be distinguished. Reciprocal crosses between streptomycin resistant and sensitive plants showed a non-Mendelian transmission of the resistance trait. Streptomycin resistance is the first selectable and maternally inherited cell organelle marker described in tomato. |
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Keywords: | Lycopersicon peruvianum Streptomycin resistance Mutagen NMU Protoplasts Chloroplast marker |
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