Correlation between pectate lyase activity and ability of diazotrophic Klebsiella oxytoca VN 13 to penetrate into plant tissues |
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| Authors: | Kovtunovych G Lar O Kamalova S Kordyum V Kleiner D Kozyrovska N |
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| Institution: | (1) Institute for Molecular Biology and Genetics of the, National Academy of Sciences, Zabolotnogo str., 150, 2522143 Kyiv, Ukraine;(2) Louisiana State University Agricultural Center, Baton Rouge, LA, USA;(3) Lehrstuhl fur Mikrobiologie Universitat Bayreuth, D-95440 Bayreuth, Germany FAX No |
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| Abstract: | Diazotrophic Klebsiella oxytoca VN 13 was able to lyse pectate, but the lytic activity of cultures grown on non-selective
media was weak, and the enzyme was located mainly inside the cells. A small fraction of the population (10-6 to 10-5) was able to grow in a selective medium with sodium polygalacturonate as sole carbon source, and produced increased amounts
of the pectinolytic enzyme pectate lyase. When wheat seeds were inoculated with these bacteria, increased levels of this enzyme
correlated with a higher rate of internal colonization of wheat roots and with stimulation of wheat growth resulting in higher
biomass. This suggests that colonization occurs via lyzed pectin layers.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | Klebsiella Endophyte Pectinase Pectate Lyase Penetration Plant |
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