Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis |
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Authors: | Upton Heather Newton Gerald L Gushiken Melissa Lo Kelly Holden Dhiraj Fahey Robert C Rawat Mamta |
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Affiliation: | Department of Biology, California State University-Fresno, Fresno, CA 93740, USA. |
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Abstract: | The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH. |
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