A membrane technique for producing protoplasts ofCryphonectria parasitica |
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| Authors: | Jyan-Chyun Jang Susan D McElreath Dr Frank H Tainter |
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| Institution: | (1) Department of Forest Resources, Clemson University, 29634-1003 Clemson, South Carolina, USA;(2) Present address: Department of Molecular Biology, Wellman 8th, Massachusetts General Hospital, 12114 Boston, MA, USA |
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| Abstract: | A method for the formation and regeneration of protoplasts of several strains of the chestnut blight fungus,Cryphonectria parasitica, is presented. The procedure utillizes cellophane membranes for growth and employs centrifugation for separation of protoplasts from hyphal fragments. Yields averaged 8.04×106 protoplasts per membrane. Regeneration frequencies were 40–50% with a soft-agar overlay. These protoplasts are suitable for use in experiments designed to determine the role of dsRNA in hypovirulence ofC. parasitica. |
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