Structure and characterization of the gene cluster of the O-antigen of <Emphasis Type="Italic">Escherichia coli</Emphasis> O49 containing 4,6-dideoxy-4-[(<Emphasis Type="Italic">S</Emphasis>-3-hydroxybutanoylamino]-D-glucose |
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| Authors: | A V Perepelov Quan Wang S N Senchenkova S D Shevelev A S Shashkov Lu Feng Y A Knirel Lei Wang |
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| Institution: | (1) Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia;(2) TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin, 300457, P. R. China;(3) Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa Street, TEDA, Tianjin, 300457, P. R. China |
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| Abstract: | An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enteropathogenic Escherichia coli O49 and studied by sugar analysis along with one- and two-dimensional 1H- and 13C-NMR spectroscopy. The following structure of the linear tetrasaccharide repeating unit of the O-polysaccharide was established: formula], where D-Qui4N(S3HOBut) stands for 4,6-dideoxy-4-(S)-3-hydroxybutanoylamino]-D-glucose and O-acetylation of GlcNAc is partial (~30%). To our knowledge, no N-(3-hydroxybutanoyl) derivative of Qui4N has been hitherto found in bacterial polysaccharides. Gene functions of the O-antigen gene cluster of E. coli O49 were assigned by bioinformatics analysis and found to correspond to the O-polysaccharide structure. Two new genes were revealed and suggested to be responsible for synthesis and transfer of the 3-hydroxybutanoyl group. |
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| Keywords: | Escherichia coli lipopolysaccharide bacterial polysaccharide structure 3-hydroxybutyrate 4-amino-4 d-glucose" target="_blank">6-dideoxy-d-glucose O-antigen gene cluster |
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