Identification of a novel metabolite in phenanthrene metabolism by the fungus Cunninghamella elegans |
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| Authors: | C E Cerniglia W L Campbell J P Freeman F E Evans |
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| Institution: | National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079. |
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| Abstract: | The metabolism of phenanthrene by the fungus Cunninghamella elegans was investigated. Kinetic experiments using 9-14C]phenanthrene showed that after 72 h, 53% of the total radioactivity was associated with a glucoside conjugate of 1-hydroxyphenanthrene (phenanthrene 1-O-beta-glucose). This metabolite was isolated by reversed-phase high-performance liquid chromatography and characterized by the application of UV absorption, 1H nuclear magnetic resonance, and mass spectral techniques. The results show that aromatic ring oxidation followed by glucosylation is a predominant pathway in the metabolism of the polycyclic aromatic hydrocarbon phenanthrene by C. elegans. |
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