Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.) |
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Authors: | Komori Toshiyuki Ohta Shozo Murai Nobuhiko Takakura Yoshimitsu Kuraya Yoshiki Suzuki Shoichi Hiei Yukoh Imaseki Hidemasa Nitta Naoto |
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Affiliation: | Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., 700 Higashibara, Toyoda, Iwata, Shizuoka 438-0802, Japan. Toshiyuki.Komori@ims.jti.co.jp |
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Abstract: | A rice nuclear gene, Rf-1, restores the pollen fertility disturbed by the BT-type male sterile cytoplasm, and is widely used for commercial seed production of japonica hybrid varieties. Genomic fragments carrying Rf-1 were identified by conducting chromosome walking and a series of complementation tests. Isolation and analysis of cDNA clones corresponding to the fragments demonstrated that Rf-1 encodes a mitochondrially targeted protein containing 16 repeats of the 35-aa pentatricopeptide repeat (PPR) motif. Sequence analysis revealed that the recessive allele, rf-1, lacks one nucleotide in the putative coding region, presumably resulting in encoding a truncated protein because of a frame shift. Rice Rf-1 is the first restorer gene isolated from cereal crops that has the property of reducing the expression of the cytoplasmic male sterility (CMS)-associated mitochondrial gene like many other restorer genes. The present findings may facilitate not only elucidating the mechanisms of male sterility by the BT cytoplasm and its restoration by Rf-1 but also isolating other restorer genes from cereal crops, especially rice. |
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Keywords: | fertility restorer Rf-1 cytoplasmic male sterility rice mitochondria pentatricopeptide repeat |
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