Cloning and characterization of the trout perforin |
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Authors: | Sofia Athanasopoulou Dimitra Marioli Angeliki Mikrou Anastasios D Papanastasiou Ioannis K Zarkadis |
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Institution: | aDepartment of Biology, School of Medicine, University of Patras, Rion 26 500 Patras, Greece |
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Abstract: | The pore-forming protein, perforin is one of the effectors of cell-mediated killing. A perforin cDNA clone was isolated from rainbow trout (Oncorhynchus mykiss) after screening of a spleen cDNA library. The full-length cDNA is 2070 bp in size, encoding for a polypeptide of 589 amino acids. The predicted amino acid sequence of the trout perforin is 64, 58 and 40% identical to those of Japanese flounder, zebrafish and human perforins, respectively. Although its membrane attack complex/perforin (MACPF) domain is conserved, trout perforin shows low homology to human and trout terminal complement components (C6, C7, C8 and C9), ranging from 19 to 26% identity. Expression analysis reveals that the trout perforin gene is expressed in the blood, brain, heart, kidney, intestine and spleen. Phylogenetic analysis of proteins which belong to the MACPF superfamily clusters the trout perforin in the same group with other known perforins. |
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Keywords: | Pore-forming toxin MACPF superfamily Perforin Evolution Rainbow trout Expression Phylogenetic analysis |
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