Microbial population and biochemical changes during production of protein-enriched fufu |
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Authors: | AO Fagbemi UJJ Ijah |
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Institution: | (1) Department of Microbiology, Federal University of Technology, P.M.B.65 Minna, Nigeria |
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Abstract: | Summary
Candida utilis strain BKT4 and Saccharomyces cerevisiae strain BKT7 isolated from burukutu (a local wine brewed from sorghum) were used to enrich fufu. During the fermentation process,
there were changes in the microbiological and biochemical characteristics of the cassava. The total viable counts increased
with increasing fermentation time while the counts of the lactics and fungi increased at the later stages of the fermentation
due to the acidity of the medium. Various bacteria (Bacillus, Staphylococcus, Klebsiella, Escherichia, Streptococcus, Lactobacillus, Leuconostoc, Corynebacterium), moulds (Penicillium, Aspergillus, Fusarium, Mucor, Rhizopus) and yeasts (Candida, Saccharomyces, Hansenula, Rhodotorula) were found to be associated with the fermentation process. The pH of the fermenting cassava increased from 4.2 to 5.7 after
72 h while the cyanide level decreased from 2.2 mg/kg to 0.7 mg/kg over the same period of fermentation. Fufu (prepared by
crushing and sieving fermenting cassava roots) enriched with 0.5 g of C. utilis strain BKT4, S. cerevisiae BKT7 and a mixed culture of the two organisms revealed a crude protein of 7.90, 6.34 and 10.0% respectively as compared to
2% protein content of the enriched fufu. There was a corresponding increase in protein content of the product as the quantity
of the enrichment yeast was increased from 0.5 to 3.0 g. The aroma of the enriched fufu was preferred to that of the commercial
fufu. Generally, good acceptability and organoleptic qualities (colour, taste, texture and aroma) of the protein enriched
fufu was best achieved within 48 h of enrichment. The results of this study suggest that fufu can be made more nutritious
with yeasts particularly Candida utilis strain BKT4 and Saccharomyces cerevisiae strain BKT7. |
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Keywords: | Cassava enrichment fufu protein yeasts |
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