Tissue-specific expression of ALA synthase-1 and heme oxygenase-1 and their expression in livers of rats chronically exposed to ethanol |
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Authors: | Zheng Jianyu Tian Qing Hou Weihong Watts John A Schrum Laura W Bonkovsky Herbert L |
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Institution: | Liver-Biliary-Pancreatic Center, Carolinas Medical Center, 1000 Blythe Boulevard, Charlotte, NC 28203, USA. jianyu.zheng@carolinashealthcare.org |
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Abstract: | 5-Aminolevulinic acid synthase-1 (ALAS1) and heme oxygenase-1 (HO-1) are the rate-controlling enzymes for heme biosynthesis and degradation, respectively. Expression of these two genes showed tissue-specific expression pattern at both mRNA and protein levels in selected non-treated rat tissues. In the livers of rats receiving oral ethanol for 10 weeks, ALAS1 mRNA levels were increased by 65%, and the precursor and mature ALAS1 protein levels were increased by 1.8- and 2.3-fold, respectively, while no changes were observed in HO-1 mRNA and protein levels, compared with pair-fed controls. These results provide novel insights into the effects of chronic ethanol consumption on hepatic heme biosynthesis and porphyrias. |
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Keywords: | ALAS1 5-aminolevulinic acid synthase-1 ALAS2 5-aminolevulinic acid synthase 2 ER endoplasmic reticulum GAPDH glyceraldehyde 3-phosphate dehydrogenase HO-1 heme oxygenase-1 LDC Lieber-DeCarli PCR polymerase chain reaction qRT-PCR quantitative real-time PCR SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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