Somatic embryogenesis in Encephalartos cycadifolius |
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Authors: | Anna K. Jäger Johannes van Staden |
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Affiliation: | (1) NU Research Unit for Plant Growth and Development, Department of Botany, University of Natal Pietermaritzburg, P/Bag X01, 3209 Scottsville, South Africa |
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Abstract: | Callus cultures of Encephalartos cycadifolius were established from zygotic embryo explants on a modified B5 medium containing 1 mg l–1 2,4-D and 1 mg l–1 kinetin. Callus was transferred to media containing various combinations of 2,4-D and kinetin for improvement of somatic embryogenesis. Somatic embryos were produced on media with several growth regulator combinations. The somatic embryos developed from proembryos, which developed long suspensors. A dicotyledonary embryo formed at the distal end of the suspensor. The embryos turned green in light. When transferred to a medium containing 1 mg l–1 ABA the somatic embryos matured. The suspensors desiccated and these embryos rooted when transferred to a medium without phytohormones.Abbreviations ABA abscisic acid - 2,4-D 2,4-dichlorophenoxyacetic acid |
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Keywords: | Cycads Encephalartos cycadifolius Gymnosperm In vitro culture Somatic embryogenesis |
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