Abstract: | Previously, we reported (Fritsch and Temin, J. Virol. 21:119-130, 1977) that infectious viral DNA was not present in spleen necrosis virus-infected stationary chicken cells. However, a stable intermediate was present in such infected stationary cells as evidenced by the appearance of infectious viral DNA shortly after serum stimulation of these cells. After serum stimulation of infected stationary cells, the infectious viral DNA appeared first in the nucleus. In contrast, in infected dividing cells the infectious viral DNA appeared first in the cytoplasm. Significantly reduced amounts of complete plus- or minus-strand viral DNAs were detected by nucleic acid hybridization in stationary chicken cells infected with spleen necrosis virus or Schmidt-Ruppin Rous sarcoma virus compared with the amounts detected in infected dividing cells. These experiments indicated that infected stationary cells did not contain complete noninfectious copies of viral DNA. Furthermore, 5-bromodeoxyuridine labeling and cesium chloride density gradient centrifugation analysis of the infectious viral DNA that appeared after serum stimulation of infected stationary cells indicated that most viral DNA synthesis occurred after addition of fresh serum. |