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Electromigration, a tool for studies on anaerobic ciliates
Authors:Stefan Wagener  Claudius K Stumm  Godfried D Vogels
Institution:Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands;Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, F.R.G.
Abstract:Abstract By applying electric current, anaerobic ciliates could be extracted from sludge samples. All the freshwater ciliates tested had one point of optimal current and voltage where they reached the highest net speed of migration in the electric field. The swimming behavior of Metopus es was tested under different current and voltage conditions. During electromigration the freshwater, marine and rumen ciliates moved from the anode to the cathode. In the anaerobic freshwater ciliates high numbersof methanogenic bacteria of different size were present. At the depth in the sludge with the highest number of Metopus minimus (660 cells ·ml?1, a peak of methane production also occurred.
Keywords:Electric field  anaerobic protozoa  methanogenic bacteria
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