Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis |
| |
Authors: | M. Zivy H. Thiellement D. de Vienne J. P. Hofmann |
| |
Affiliation: | (1) Laboratoire de Génétique des Systèmes Végétaux, G.I.S. Moulon, F-91190 Gif-sur-Yvette, France |
| |
Abstract: | Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between Chinese Spring and Selkirk is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated. |
| |
Keywords: | Triticum aestivum 2D electrophoresis Developmental genetics Cytoplasmic inheritance Genetic regulation |
本文献已被 SpringerLink 等数据库收录! |