| 树脂吸附法分离瓜果腐霉毒素中的除草活性物质 |
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| 引用本文： | 张利辉,王毅靖,张金林,董金皋. 树脂吸附法分离瓜果腐霉毒素中的除草活性物质[J]. 微生物学通报, 2008, 35(9): 1505-1509 |
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| 作者姓名： | 张利辉 王毅靖 张金林 董金皋 |
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| 作者单位： | 河北农业大学植物保护学院,保定,071001 |
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| 基金项目： | 河北省自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 本试验以对禾本科杂草具有除草活性的瓜果腐霉菌株PAM1为试验材料,研究了X-5、AB-8,H-103和S-8等4种大孔吸附树脂对其除草活性物质的吸附性能,结果表明X-5树脂能够较好地吸附该除草活性物质.选用50%乙醇、95%乙醇、乙酸乙酯和丙酮4种溶剂为供试洗脱溶剂,采用2种洗脱方案,对其最佳洗脱溶剂进行了选择,生长抑制作用和种子萌发试验结果表明,以方案1洗脱为最佳.
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| 关 键 词： | 瓜果腐霉病菌 除草活性 树脂柱层析 |
Colophony Adsorbing Isolating Herbicidal Substance from Mycotoxin Produced by Pythium aphanidermatum |
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| ZHANG Li-Hui,WANG Yi-Jing,ZHANG Jin-Lin and DONG Jin-Gao. Colophony Adsorbing Isolating Herbicidal Substance from Mycotoxin Produced by Pythium aphanidermatum[J]. Microbiology China, 2008, 35(9): 1505-1509 |
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| Authors: | ZHANG Li-Hui WANG Yi-Jing ZHANG Jin-Lin DONG Jin-Gao |
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| Affiliation: | College of Plant Protection of Agricultural University of Hebei, Baoding 071001;College of Plant Protection of Agricultural University of Hebei, Baoding 071001;College of Plant Protection of Agricultural University of Hebei, Baoding 071001;College of Plant Protection of Agricultural University of Hebei, Baoding 071001 |
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| Abstract: | Four kinds of colophony were used to isolate the herbicidal substance from the toxin produced by isolate PAM1 of Pythium aphanidermatum, which had herbicidal activity to Digtaria sanguinealis L and the results showed X-5 was the best one for absorbing herbicidal substance among the four kinds of colophony used. Four solvents including 50% ethanol, 95% ethanol, ethyl acetate and acetone were used as eluting solvent and 2 procedures were tested, and the results of growth inhibition and seed germination indicated that the best eluting procedure was procedure 1. |
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| Keywords: | Pythium aphanidermatum Herbicidal activity Colophony |
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